A new assay procedure for the enzymes and studies on their intracellular localization
Shaking introduces air bubbles and mechanical stress that can degrade the compound
At the end of the experiment, MTT assay, Annexin V/Hoechst 33342 staining, flow cytometry, and cell lysate collection were performed
Subsequent randomized controlled data demonstrated that RBCs stored longer periods (roughly 35 weeks) were non-inferior to fresher RBCs (e.g., less than 7 days) in ICU and cardiac surgery patients among others (Steiner et al., 2015), but this has not been carefully studied in trauma
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