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Slides were washed with PBS-Tween, then incubated with secondary antibodies donkey anti-rabbit IgG (H+L) highly cross-absorbed Alexa Fluor Plus 647 (A32795, 1:500), donkey anti-rat IgG (H+L) highly cross-absorbed Alexa Fluor Plus 647 (A48272, 1:500), and donkey anti-goat IgG (H+L) cross-absorbed Alexa Fluor 555 (A-21432, 1:500) for 45 minutes at room temperature

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